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A Punnett foursquare is a square diagram used to predict the genotypes of an offspring. The genotype is the actual genetic makeup of an organism, usually written in alleles. Humans have two alleles for every autosomal factor in our body. A typical monohybrid cross follows one cistron and can be easily observed in a 2 ten 2 Punnett foursquare. If you wanted to track two genes together a 4 x 4 Punnett foursquare volition come in handy. Simply, instead of filling in 16 boxes, in that location is a quicker fashion to find out the genotypes using the forked line method.

  1. 1

    Highlight clues in the problem. Information technology is important to decipher what the problem is asking. Brand sure to highlight what the dissimilar alleles produce because information technology can go disruptive.

  2. 2

    Write downwards the genotypes for both parents.

    • Heterozygous for a gene means that one allele is ascendant and i allele is recessive.
    • Homozygous means that both alleles are either ascendant or recessive.

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  3. 3

    Depict 2 2x2 Punnett squares. Kickoff, draw a large foursquare and divide it into 4 equal squares. Repeat this step one more time. You will be crossing each cistron from each parent separately. The cistron for size and the gene for color will go in their ain 2x2 Punnett square.

  4. 4

    Label the Punnet square. The female parent's genotype, and the sides of the Punnett square with the father'southward genotype. Remember that an individuals genotype for a dihybrid cross has four alleles. These 4 alleles make up ii different genes.

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  1. 1

    Perform a Punnet square cross two times. Accept the mother first allele write it into the ii boxes below. Exercise this step again for the mother'due south 2nd allele. And so have the male parent's starting time allele and write it into the two boxes to its right. Practise this footstep once again for the begetter's 2nd allele. Once ane punnet square is total do it once more for the other cistron. Information technology is important to write the dominant allele first in the box when crossing the two alleles. "Rr" is preferred over "rR"

  2. 2

    List downwardly the genotypes for each cross. The two letters in each box are the genotypes.

  3. 3

    Summate how frequent each genotype shows upward in both Punnett square. Answers should be written in fraction course. The numerator for each genotype should be somewhere from 1-4 and the denominator should exist 4 for each genotype.

  4. iv

    List downwards the new three genotype frequencies you lot establish from the get-go genetic cross (Punnett square #1). Make sure to write these numbers in a higher place ane another, leaving about 2 or 3 inches of space between them.

  5. 5

    Describe three arrows after each of the three frequencies. One arrow should point slightly up, one horizontal, and the other slightly downwards. This is where the phrase forked line method is derived from. It should look like a fork at this bespeak. A total of ix arrows should be fatigued.

  6. 6

    List downwardly the other three possible genotypic frequencies for the 2nd Punnett square on each terminate of an arrow. There should be a total of 9 genotypic frequencies written, but they are only 3 different frequencies.

    • Make sure this step looks the same after each of the 3 separate genotypic frequencies.
  7. seven

    Draw a horizontal arrow afterward the genotypic frequencies you wrote down. There should exist 9 arrows.

  8. 8

    Combine the genotype from the beginning column and second column and write them afterwards the concluding arrow. Do this footstep 9 times until all arrows accept a iv letter of the alphabet genotype.

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  1. 1

    Write the genotypic frequency for each of the 4 letter genotype. To find this multiply the frequency in the first column to the frequency in the 2nd column.

    • A calculator tin can be used or just multiply the numerator separately and the denominator separately and turn information technology into a fraction. The denominator should be out of 16 because (4 * 4= 16).
  2. two

    Grouping the genotypic ratios of the genotypes that produce the same looking offspring. This is to find the phenotypic ratios. There are 9 unlike genotypes with 4 different concrete combinations possible. At that place should exist 4 unlike groups of phenotypic ratios.

  3. 3

    Detect the sum of the genotypic ratios that produce the aforementioned phenotype.

    • The number should be in fraction class with the denominator of 16.
  4. 4

    Write down the phenotypic ratio in standard form for the entire cross. The phenotypic ratios for this cross are 9/sixteen, 3/16, 3/16, 1/16. To simplify these numbers, you would write nine:three:3:1.

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  • If 2 heterozygous parents are crossing then the ratio volition always be 9:3:3:1.

  • A homozygous dominant parent crossed with a homozygous recessive parent so the all the offspring will be heterozygous.

  • These crosses simply works for genes that follow independent assortment.

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